Peptide Reconstitution: A Step-by-Step Lab Guide

Lyophilized (freeze-dried) peptides ship as a stable powder. Proper reconstitution is essential to preserve the peptide's integrity.

Step 1: Choose the right solvent

• Bacteriostatic water (0.9% benzyl alcohol) — standard for short-term storage
• Sterile water — for single-use applications
• Acetic acid solution — for acidic peptides with poor water solubility

Step 2: Sanitize

• Wipe the vial septum with an alcohol pad
• Use a sterile syringe and needle

Step 3: Add solvent slowly

• Do not apply solvent directly onto the powder — aim at the side of the vial
• Allow the solvent to run down the wall of the vial

Step 4: Swirl gently

• Do not vortex or shake aggressively
• Gentle swirling allows 5 minutes for full dissolution

Step 5: Inspect

• The solution should be clear
• Cloudiness suggests contamination or aggregation

Step 6: Label

• Reconstitution date, concentration, solvent, and your initials

Storage after reconstitution

• 2-8C refrigerated: 14-30 days for most peptides
• -20C frozen: longer storage, but avoid repeated freeze-thaw cycles
• Aliquot before freezing if using across many sessions

Learn more about peptide storage ->

FAQ

How do you reconstitute a peptide?

Add bacteriostatic water slowly to the side of the vial (not directly onto the powder), swirl gently for 5 minutes, and inspect for clarity.

What solvent should I use?

Bacteriostatic water (0.9% benzyl alcohol) is standard for most peptides.