Methylene Blue Solution: molecular data, studies & sources

Methylene Blue Solution is catalogued here as a research compound. This profile brings together the molecular source record, selected indexed publications and available supplier comparisons. Evidence for a named substance does not authenticate a commercial product.

Compiled by PeptideTech Editors · Sources checked 2026-09-21 · Selected bibliography, not a systematic review

Molecular formula, weight & structure

Molecular formula (MF): C16H18ClN3S
Molecular weight (MW): 319.9 g/mol
PubChem compound ID: 6099
Record title: Methylene Blue

Values describe the selected PubChem molecular entity. Counterions, salt forms, hydration and sequence changes can alter the supplied material. Confirm the exact identity against the supplier specification.

Source: PubChem CID 6099 · retrieved 2026-09-21

PubChem 2D molecular structure for Methylene Blue Solution, CID 6099 — 2D depiction from PubChem. Open to inspect the full image. This drawing represents the selected record, not a vendor batch test.

Chemical identifiers and source data

IUPAC name: [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium chloride
InChIKey: CXKWCBBOMKCUKX-UHFFFAOYSA-M
SMILES: CN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.[Cl-]

Machine-readable PubChem source

Human and clinical studies

Selected human-study records are shown with their indexed or reviewed study type. Check the original article for the intervention, formulation and controls; the molecule can also be a comparator or measured variable.

Human study · PubMed trial index
Early adjunctive methylene blue in patients with septic shock: a randomized controlled trial.
Ibarra-Estrada M, Kattan E, Aguilera-González P et al. · Critical care (London, England) · 2023
PMID 36915146 · DOI: 10.1186/s13054-023-04397-7
Human study · PubMed trial index
Infusion of methylene blue in human septic shock: a pilot, randomized, controlled study.
Kirov MY, Evgenov OV, Evgenov NV et al. · Critical care medicine · 2001
PMID 11588440 · DOI: 10.1097/00003246-200110000-00002

Preclinical research, reviews & other publications

Animal studies, laboratory experiments, reviews and other indexed papers answer different questions. A review is secondary evidence; a case report does not estimate how often an outcome occurs.

Review · secondary evidence
The Potentials of Methylene Blue as an Anti-Aging Drug.
Xue H, Thaivalappil A, Cao K et al. · Cells · 2021
PMID 34943887 · DOI: 10.3390/cells10123379
Review · secondary evidence
Methylene-blue-associated encephalopathy.
Sweet G, Standiford SB · Journal of the American College of Surgeons · 2007
PMID 17324781 · DOI: 10.1016/j.jamcollsurg.2006.12.030
Research publication · design requires review
Methylene blue: revisited.
Ginimuge PR, Jyothi SD · Journal of anaesthesiology, clinical pharmacology · 2010
PMID 21547182

Methylene Blue Solution supplier sources

Scientific sources describe molecules and experiments. The commercial links below identify recorded supplier packages. A publication or chemical database entry does not verify the seller, purity, sterility or contents of a lot. Some links are affiliate links; see our disclosure.

Compare all Methylene Blue Solution sizes, forms and supplier prices →

Supplier	Recorded packages	Example source listing
Amino USAUSD	10mg-per-mL-30mL Solution1 recorded offers	10mg-per-mL-30mL Solution · $18.00Checked Sep 16, 2026Supplier listing

Reference sources & further reading

PubChem compound record 6099 — chemical identity and structure.
Wikipedia: Methylene blue — Background encyclopedia; not a primary clinical source.

The citations above link directly to publication records. Encyclopedia links are background reading and are not used to establish clinical effectiveness.

Search for additional research

These links run searches; their results have not been individually reviewed and can include unrelated compounds.

Research information is not a dosing guide or a recommendation for personal use. Study findings are specific to the formulation, population and methods tested. Read our source and correction policy.